AF568 mal（Alexa Fluor 568 Maleimide），用于荧光共振能量转移（FRET）研究

试剂介绍

AF568 mal（Alexa Fluor 568 Maleimide）是一种基于Alexa Fluor 568荧光染料与马来酰亚胺（Maleimide）基团共价结合的复合型荧光标记试剂。其核心功能是通过马来酰亚胺基团与含巯基（-SH）的生物分子（如蛋白质、多肽）发生特异性反应，形成稳定的硫醚键，从而实现荧光标记，适用于生物分子示踪及荧光共振能量转移（FRET）研究。

AF568 mal ( Alexa Fluor 568 Maleimide ) is a composite fluorescent labeling reagent based on the covalent binding of Alexa Fluor 568 fluorescent dye and Maleimide group. Its core function is to form a stable thioether bond through the specific reaction of maleimide group with thiol-containing ( -SH ) biomolecules ( such as proteins, peptides ), so as to achieve fluorescence labeling, which is suitable for biomolecule tracking and fluorescence resonance energy transfer ( FRET ) research.

英文名称：AF568 mal、AF568 Maleimide、Alexa Fluor 568 Maleimide、Alexa Fluor 568 mal

中文名称：AF568马来酰亚胺、Alexa Fluor 568马来酰亚胺、AF568马来酰亚胺酯

外观：橙色至深红色固体

分子式：C₄₄H₄₅N₄NaO₁₂S₂

分子量：853.02

激发波长579 nm

发射波长603 nm

规格：5mg、10mg、25mg

纯度：95%+

储存：-20℃以下冷冻干燥保存，避免反复冻融

供应商：陕西新研博美生物科技有限公司

特点分析

双功能集成：同时具备Alexa Fluor 568的高亮度荧光特性与马来酰亚胺的巯基反应专一性，无需额外交联剂即可实现定向标记。

光稳定性优势：相比传统荧光素（如FITC），AF568 mal在持续光照下光漂白速率降低40%，适合长时间成像实验。

pH兼容性：在弱酸性至中性环境（pH 5-8）中保持标记效率稳定，适用于多种细胞内标记场景。

Bifunctional integration : It has the high brightness fluorescence characteristics of Alexa Fluor 568 and the thiol reaction specificity of maleimide, and can achieve directional labeling without additional crosslinking agent.

Light stability advantage : Compared with traditional fluorescein ( such as FITC ), the photobleaching rate of AF568 mal is reduced by 40 % under continuous illumination, which is suitable for long-term imaging experiments.

pH compatibility : The labeling efficiency is stable in a weakly acidic to neutral environment ( pH 5-8 ), which is suitable for a variety of intracellular labeling scenarios.

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AF568 mal（Alexa Fluor 568 Maleimide），用于荧光共振能量转移（FRET）研究

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